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51.
Naoaki Tsutsui Tsuyoshi Ohira Tomoyuki Okutsu Junpei Shinji Sun-Hye Bae Bong Jung Kang Marcy N. Wilder 《Fisheries Science》2013,79(3):357-365
The cDNA encoding a precursor of Liv-SGP-G, the most abundant crustacean hyperglycemic hormone-family peptide in the sinus gland of the whiteleg shrimp Litopenaeus vannamei, was cloned by RT-PCR coupled with 5′- and 3′-RACE. The determined cDNA sequence consisted of 621 nucleotides comprising a 69-bp 5′-untranslated region, a 357-bp open reading frame, and a 195-bp 3′-untranslated region. The deduced sequence of 72 amino acid residues corresponding to mature Liv-SGP-G was completely identical to that of natural Liv-SGP-G, determined in our previous study. The recombinant Liv-SGP-G was thereafter heterologously expressed in Escherichia coli, and its vitellogenesis-inhibiting activity in ex vivo cultured ovarian fragments was examined. The recombinant peptides inhibited vitellogenin gene expression with almost the same efficacy as that of natural Liv-SGP-G purified from sinus glands. 相似文献
52.
Tomohiro Yonezawa Kohei Sato Mona Uchida Naoaki Matsuki Atusi Yamazaki 《Animal Science Journal》2017,88(1):195-200
Contagious yawning is triggered by others yawning, and it has previously been reported in humans, primates and several experimental and companion mammals. Whereas it might be a response to an innate releasing mechanism, contagious yawning is also considered to involve emotional contagion. Here, we demonstrate that sheep, the animal model of livestock animals, also experience contagious yawning. Twelve adult castrated Corriedale sheep were used in this study. Pairs of sheep were adjacently restrained with or without a wooden divider panel to shield them from viewing the other. Their behaviors were video‐recorded for 3 days in each condition. Sheep yawned 2.0 ± 1.1 and 1.2 ± 1.1 times/day/head in the unshielded and shielded conditions, respectively. Unshielded restrained sheep yawned within 1 min after the other one 11.1% of the time, while shielded pairs did not exhibit contagious yawning. Rumination was also highly synchronized under the unshielded condition. These data reveal that contagious yawning and behavioral synchronicity occur in ruminants like sheep, making them a suitable animal model to investigate contagious yawning and the underlying mechanism. 相似文献
53.
Yamada K Hirotsu T Matsuki M Butcher RA Tomioka M Ishihara T Clardy J Kunitomo H Iino Y 《Science (New York, N.Y.)》2010,329(5999):1647-1650
Population density-dependent dispersal is a well-characterized strategy of animal behavior in which dispersal rate increases when population density is higher. Caenorhabditis elegans shows positive chemotaxis to a set of odorants, but the chemotaxis switches from attraction to dispersal after prolonged exposure to the odorants. We show here that this plasticity of olfactory behavior is dependent on population density and that this regulation is mediated by pheromonal signaling. We show that a peptide, suppressor of NEP-2 (SNET-1), negatively regulates olfactory plasticity and that its expression is down-regulated by the pheromone. NEP-2, a homolog of the extracellular peptidase neprilysin, antagonizes SNET-1, and this function is essential for olfactory plasticity. These results suggest that population density information is transmitted through the external pheromone and endogenous peptide signaling to modulate chemotactic behavior. 相似文献
54.
Manuel Albert V. Tu Phan Thi Cam Tsutsui Naoaki Yoshimatsu Takao 《Fisheries Science》2020,86(1):27-33
Fisheries Science - The embryonic developmental response of two abalone species (disk abalone Haliotis discus discus, giant abalone Haliotis gigantea) to a drop in salinity with different exposure... 相似文献
55.
Phan Thi Cam Tu Manuel Albert Valdish Tsutsui Naoaki Yoshimatsu Takao 《Fisheries Science》2020,86(1):119-125
Fisheries Science - Heavy rainfall can reduce salinity and increase turbidity in coastal waters, creating stressful conditions for the organisms found there, especially for the early stages of... 相似文献
56.
With data sampled from 2003 to 2006, this study presents the effects of temperature, food availability (C), and body size on the somatic growth rate [^(g)] \hat{g} (mm day−1) and on the size-specific growth rates [^(G)] \hat{G} (day−1) of larval Japanese anchovy Engraulis japonicus caught in Hiuchi-nada, Seto Inland Sea, Japan, a semi-enclosed narrow sea. C was calculated using an individual-based model, which is an encounter model between copepods and anchovy where the anchovy
resulted in a successful capture of the copepod. [^(g)] \hat{g} decreased with increasing anchovy length, but increased with increasing C. No significant relationship was found between [^(g)] \hat{g} and temperature. [^(G)] \hat{G} decreased with increasing temperature, but anchovy weight, on the other hand, increased with increasing C. In the literature, the mechanism of growth determination for larvae of pelagic fish species is considered to be primarily
determined by sea temperature and secondarily by food availability. However, [^(g)] \hat{g} and [^(G)] \hat{G} were found to be dependent on C rather than temperature. [^(G)] \hat{G} was dependent on anchovy weight rather than C and temperature. For forecasting the number of days required for development from an arbitrary pre-recruitment size to the
recruitment size, we address not only temperature but also food availability and the size effect when considering the mechanism
of growth determination for larvae. 相似文献
57.
Purpose To report two cases of corneal squamous cell carcinoma (SCC) in dogs. Methods Corneal tumors were resected by superficial keratectomy in two cases. Immunohistochemistry of the corneal tissues was performed using anti‐p53 antibody. Results The prominent features of the cases were a clinical history of pigmentary keratitis and chronic keratitis. In each case, a corneal mass was surgically removed with a superficial keratectomy and histologically diagnosed as corneal SCC. Both masses were negative for p53. To reduce chronic corneal irritation, 0.1% hyaluronate sodium ophthalmic solution was applied. After more than 15 months of postsurgical follow‐up there has been no recurrence of either neoplasm. Conclusion and discussion Chronic corneal irritation was suspected as the primary etiology for the corneal SCC. Appropriate surgical removal of the mass and subsequent conservative treatment for keratitis provided effective therapy in these two cases. 相似文献
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60.
Kakizaki T Yokoyama Y Natsuhori M Karasawa A Kubo S Yamada N Ito N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(4):361-365
Pharmacokinetics (PK) of probenecid including plasma probenecid concentrations, in vitro plasma protein binding properties, and in vivo PK parameters were determined in dogs. Probenecid concentrations were best determined by HPLC, which showed good linearity and good recovery with simple plasma preparation. The quantification limit of probenecid was approximately 50 ng/ml at S/N ratio = 3, by simple procedure with HCl and methanol treatment. Probenecid showed two types of binding characteristics, i.e., high-affinity with low-capacity and low-affinity with high-capacity binding. This result indicated 80-88% of probenecid was bound to plasma protein(s) at observed concentrations (< 80 microg/ml) in vivo at an intravenous dose of 20 mg/kg. Plasma probenecid concentration-time profile following i.v. administration in dogs showed biphasic decline and well fitted a two-compartment open model. The total body clearance was 0.34 +/- 0.04 ml/min/kg, volume of distribution at steady-state was 0.46 +/- 0.07 l/kg, elimination half-life was 18 +/- 6 hr, and mean residence time (MRT) was 23 +/- 6 hr. Since probenecid has been known as a potent inhibitor of renal tubular excretion of acidic drugs and highly binds to plasma proteins, our observation in relation to plasma protein binding and PK parameters will serve as the basic information concerning drug-drug interactions in dogs and in other mammalian species. 相似文献